Emergence of effective temperatures in an out-of-equilibrium model of RNA folding

Marco Ancona, Alessandro Bentivoglio, Michele Caraglio, Giuseppe Gonnella, Alessandro Pelizzola

Published 2020-09-23Version 1

We investigate the possibility of extending the notion of temperature in a stochastic model for the RNA/protein folding driven out of equilibrium. We simulate the dynamics of a small RNA hairpin subject to an external pulling force, which is time-dependent. First, we consider a fluctuation-dissipation relation (FDR), that extends the fluctuation-dissipation theorem in nonequilibrium contexts. We verify that various effective temperatures values can be obtained in a range of parameters and for different observables, only when the slowest intrinsic relaxation timescale of the system regulates the dynamics of the system. Then, we introduce a different nonequilibrium temperature, which is defined from the rate of heat exchanged with a weakly-interacting thermal bath. Notably, this `kinetic' temperature can be defined for any frequency of the external switching force. We also discuss and compare the behavior of these two emerging parameters, by discriminating the time-delayed nature of the FDR temperature from the instantaneous character of the kinetic temperature.